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My CartReactivity：Monkey,Human
Application：ChIP,IP,ICC,WB,IF
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：2547
Host：Mouse
Qty：
Size：
| Name | Anti-XRCC6 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 7B1-E12-G4 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ChIP 1:20. ICC 1:50-200. |
| Target | XRCC6 |
| Background | The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008] |
| Swiss Prot | P12956 |
| Gene ID | 2547 |
| Subcellular Location | Chromosome Nucleus |
| Immunogen | Recombinant protein of human XRCC6 |
| Calculated MW | 70kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Monkey Human |
| Application | ICC ChIP IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with XRCC6 antibody diluted at 1:1000;Lane: A549,HeLa,COS7,K562.
Immunofluorescence analysis of HeLa cells using XRCC6 antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using XRCC6 antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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