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My CartReactivity：Mouse,Rat,Human
Application：IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1499
Host：Mouse
Qty：
Size：
| Name | Anti-CTNNB1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 4F2 |
| Dilution Ratio | WB 1:1000-2000. IHC 1:200-500. |
| Target | CTNNB1 |
| Background | The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development. |
| Swiss Prot | P35222 |
| Gene ID | 1499 |
| Subcellular Location | Cell junction Cell membrane Cytoplasm Nucleus adherens junction centrosome cytoskeleton microtubule organizing center spindle pole |
| Immunogen | Recombinant Protein |
| Calculated MW | 85kDa |
| Purification Method | Saturated ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CTNNB1 antibody diluted at 1:2000 , lane1-4: Hela, 293T, Mouse Liver Tissue, Rat Liver Tissue
Immunohistochemistry of paraffin-embedded Human Lung cancer with CTNNB1 antibody diluted at 1:500
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