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My CartReactivity：Human,Rat,Mouse
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：10971
Host：Rabbit
Qty：
Size：
| Name | Anti-YWHAQ Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. |
| Target | YWHAQ |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. |
| Swiss Prot | P27348 |
| Gene ID | 10971 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human YWHAQ |
| Public Immunogen Range | 135-185/245aa |
| Calculated MW | 27kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with YWHAQ antibody diluted at 1:2000;Lane: A549,HeLa,A431,NIH/3T3,SW480,HT29,SHSY5Y,Mouse kidney,Mouse brain,Rat brain.
Immunohistochemistry of paraffin-embedded Human ovarian cancer with YWHAQ antibody diluted at 1:200.
Immunohistochemistry of paraffin-embedded Human prostate cancer with YWHAQ antibody diluted at 1:200.
Immunofluorescence analysis of HeLa cells using YWHAQ antibody diluted at 1:100.
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