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My CartReactivity：Human,Mouse
Application：WB,IF,IHC,IP
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2145
Host：Rabbit
Qty：
Size：
| Name | Anti-EZH1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. IP 1:50-500. |
| Target | EZH1 |
| Background | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Swiss Prot | Q92800 |
| Gene ID | 2145 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human EZH1 |
| Public Immunogen Range | 50-100/747aa |
| Calculated MW | 69/77/85kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | IP WB IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EZH1 antibody diluted at 1:2000;Lane: HT-29,SW480,HepG2,K562,MCF7,HeLa,293T.
Immunohistochemistry of paraffin-embedded Human renal cancer with EZH1 antibody diluted at 1:200.
Immunofluorescence analysis of A431 cells using XPR1 antibody diluted at 1:100.
Immunoprecipitation analysis of Hela extracts using EZH1 antibody(IP:3-12ug;WB:1:1000).Control IgG: Normal Rabbit IgG.
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