5'-nucleotidase（5'-NT）Activity Assay Kit

Determination of Significance：

5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus.

Measurement Principle：

5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus.

Self Provided：

Balance, Spectrophotometer/Microplate reader, desktop centrifuge, cryogenic centrifuge, constant temperature water bath/constant temperature incubator, micro glass cuvette/96 well plate, transferpettor, mortar/homogenizer, ice, distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.