Determination of Significance：

5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus.

Measurement Principle：

5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus.

Self Provided：

Balance, Spectrophotometer/Microplate reader, desktop centrifuge, cryogenic centrifuge, constant temperature water bath/constant temperature incubator, micro glass cuvette/96 well plate, transferpettor, mortar/homogenizer, ice, distilled water.

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