Determination of Significance:
DPPH free radical is a very stable nitrogen-centered free radical. It is one of the important indicators of the sample's antioxidant capacity and is widely used in the research of antioxidant foods, health products and pharmaceuticals.
Measurement Principle:
The DPPH radical has a single electron, and its alcohol solution is purple, with strong absorption at 515 nm. When an antioxidant present, DPPH free radicals are cleared, the solution color becomes lighter, and the absorbance at 515 nm decreases. Within a certain range, the change in absorbance is directly proportional to the degree of free radical removal. In this kit, the ability of the sample to remove DPPH free radicals is reflected by the degree of decrease in absorbance.
Self Provided:
Spectrophotometer, water bath, centrifuge, 1mL glass cuvette, motor/grinder, absolute ethanol, oven, 30-50 mesh sieve and distilled water.
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