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Determination of Significance：
Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained.
Measurement Principle：
Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained.
Self Provided：
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogeniser, ice and distilled water.
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