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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-VCL Polyclonal AntibodyReactivity：Human,Rat,Mouse
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：7414
Host：Rabbit
Qty：
Size：
| Name | Anti-VCL Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:100-300. IF 1:50-200. |
| Target | VCL |
| Background | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. |
| Swiss Prot | P18206 |
| Gene ID | 7414 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human VCL |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 25/117/124kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with VCL antibody diluted at 1:200.
Immunofluorescence analysis of NIH3T3 cells using VCL antibody diluted at 1:50.
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MiR-29a-3p promotes nasal epithelial barrier dysfunction via direct targeting of CTNNB1-VCL module in allergic rhinitis
Click to check >>Author：Na Wang, Pu Li, Junqi Liu, Zhenlin Wang
IF：5.7140
Publish_to：INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：37262956
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