PEG6000
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Cat.No：IP9110 Solarbio
CAS：25322-68-3
Storage：Store at RT，2 years
Appearance：White to off-white Solid
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Colloidal Gold Reagent
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Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
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Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
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Pipette & Dispenser & Pipette Dispensing Instrument
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Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| CAS | 25322-68-3 |
| Name | PEG6000 |
| Molecular Formula | HO(CH2CH2O)nH |
| Molecular Weight | ≈6000 |
| Solubility | Soluble in Water ≥100mg/mL |
| Appearance | White to off-white Solid |
| Storage | Store at RT，2 years |
| EC | EINECS 203-473-3 |
| MDL | MFCD00081839 |
| Background | Polyethylene glycol is a kind of high molecular polymer, which can be used in the research of drug sustained release and immobilized enzyme carrier, surface modification of medical polymer materials and so on. |
| Unit | Bottle |
| Specification | 100g |
Examples of using this product（for reference only）
In Vitro:
Lentivirus production and generation of clonal cell lines:
Concentrated shRNA lentiviruses were obtained by precipitating viral containing supernatants with PEG6000 (Solarbio).
Reference：
Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. Real-time imaging of RNA polymerase I activity in living human cells. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. PMID: 36282216; PMCID: PMC9606689.
Lentivirus production and generation of clonal cell lines:
After 60 h of transfection, virus supernatant was harvested and mixed with PEG 6000 (Solarbio) in a ratio of 2:1. The virus was then concentrated after 12 h storage at 4℃. Finally, the virus precipitation was resuspended in PBS and stored at -80℃.
Reference：
Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B. Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions. J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. PMID: 36453882; PMCID: PMC9769385.
Plant:
The 300 mM NaCl (Solarbio, Beijing, China) solution and 10% PEG6000 (Solarbio, Beijing, China) solution were used to simulate salt stress and drought stress, respectively. The leaves and roots were collected at 0, 3, 6, 12 and 24 h under different abiotic stresses, and the samples were quick-frozen in liquid nitrogen. To analyze the tissue-specific expression level of GhTIP1;1-like in TM-1, roots, stems and leaves were collected from seedlings, and buds, sepals, petals, stamens and fiber samples.
Reference：
Cheng G, Wang M, Zhang L, Wei H, Wang H, Lu J, Yu S. Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis. Int J Mol Sci. 2022 Jan 25;23(3):1361. doi: 10.3390/ijms23031361. PMID: 35163287; PMCID: PMC8836057.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Cheng G, et al. Int J Mol Sci. 2022 Jan 25;23(3)1361.
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