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Your Position: Home > Small Molecule Compounds > Cosolvent & Solubilizer > PEG6000
PEG6000

CAS:25322-68-3

Appearance:White to off-white solid

Storage:RT, valid for 2 years.

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PEG6000
Cat.No：
IP9110
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
IP9110-100g Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A


Examples of using this product（for reference only）

In Vitro:

Lentivirus production and generation of clonal cell lines:

Concentrated shRNA lentiviruses were obtained by precipitating viral containing supernatants with PEG6000 (Solarbio).

Reference：

Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. Real-time imaging of RNA polymerase I activity in living human cells. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. PMID: 36282216; PMCID: PMC9606689.

Lentivirus production and generation of clonal cell lines:

After 60 h of transfection, virus supernatant was harvested and mixed with PEG 6000 (Solarbio) in a ratio of 2:1. The virus was then concentrated after 12 h storage at 4℃. Finally, the virus precipitation was resuspended in PBS and stored at -80℃.

Reference：

Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B. Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions. J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. PMID: 36453882; PMCID: PMC9769385.

Plant:

The 300 mM NaCl (Solarbio, Beijing, China) solution and 10% PEG6000 (Solarbio, Beijing, China) solution were used to simulate salt stress and drought stress, respectively. The leaves and roots were collected at 0, 3, 6, 12 and 24 h under different abiotic stresses, and the samples were quick-frozen in liquid nitrogen. To analyze the tissue-specific expression level of GhTIP1;1-like in TM-1, roots, stems and leaves were collected from seedlings, and buds, sepals, petals, stamens and fiber samples.

Reference：

Cheng G, Wang M, Zhang L, Wei H, Wang H, Lu J, Yu S. Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis. Int J Mol Sci. 2022 Jan 25;23(3):1361. doi: 10.3390/ijms23031361. PMID: 35163287; PMCID: PMC8836057.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Cheng G, et al. Int J Mol Sci. 2022 Jan 25;23(3)1361.
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