Citric acid

Examples of using this product（for reference only）

HPLC

HPLC was performed using an equipped with a DAD detector and a C18 (250 mm × 4.6 mm; 5 μm). The mobile phase included 0.2% phosphoric acid plus 0.4% sodium 1-heptanesulfonate (A) and methanol (B) at a flow speed of 1.0 ml/min in the condition of a column temperature of 30℃. The detection wavelength was set at 214 nm. The reference standard sample injection volume was 10 μl. The gradient elution was as follows: 0–5 min (100–100% A, 0%–0% B), 5–60 min (100–60% A, 0%–40% B), 60–120 min (60–30% A, 40–70% B), 120–125 min (30–30% A, 70–70% B), 125–125.1 min (30–100% A, 70–0% B), 125.1–132 min (100–100% A, 0–0% B). The chromatographic data and peak area scores were collected and analyzed using ChemStation software.

来源文献：Duan Q, Liu T, Huang C, Shao Q, Ma Y, Wang W, Liu T, Sun J, Fang J, Huang G, Chen Z. The Chinese Herbal Prescription JieZe-1 Inhibits Membrane Fusion and the Toll-like Receptor Signaling Pathway in a Genital Herpes Mouse Model. Front Pharmacol. 2021 Sep 24;12:707695. doi: 10.3389/fphar.2021.707695. PMID: 34630083; PMCID: PMC8497740.

GC-MS

with minor modification, using an Agilent 7890 gas chromatograph (Agilent Technologies, Santa Clara, CA), equipped with an Agilent 5975C series mass spectrometer. The volatile compounds were separated using a DBWax (30 m × 0.25 mm i.d. × 0.25 μm; Agilent Technologies) fused silica capillary column. Helium was used as the carrier gas and it was circulated at a rate of 1 mL/min in constant flow mode. The oven temperature was held at 35 ℃ for 2 min, ramped at a rate of 2 ℃/min up to 45 ℃, then ramped to 130 ℃ at the rate of 5 ℃/min, and finally ramped to 225 ℃ at a rate of 10 ℃/min for 5 min.

来源文献：Pan X, Zhang W, Lao F, Mi R, Liao X, Luo D, Wu J. Isolation and identification of putative precursors of the volatile sulfur compounds and their inhibition methods in heat-sterilized melon juices. Food Chem. 2021 May 1;343:128459. doi: 10.1016/j.foodchem.2020.128459. Epub 2020 Oct 23. PMID: 33158672.

HPLC

The content of LMWOAs (oxalic, citric, malic, tartaric, formic,succinic, malonic,lactic and acetic acids) ware analyzed by high-performance liquid chromatography with a C18 column (250 mm × 4.6 mm × 5 μm). HPLC conditions were as follows: mobile phase, 20 mM KH2PO4 (pH 2.2) and methanol (98:2, v/v); flow rate, 1 mL/min; temperature, 25 ℃;UV detection wavelength, 210 nm.

来源文献：Li X, Ma H, Li L, Gao Y, Li Y, Xu H. Subcellular distribution, chemical forms and physiological responses involved in cadmium tolerance and detoxification in Agrocybe Aegerita. Ecotoxicol Environ Saf. 2019 Apr 30;171:66-74. doi: 10.1016/j.ecoenv.2018.12.063. Epub 2018 Dec 28. PMID: 30597318.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.