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Your Position: Home > Small Molecule Compounds > Inhibitors & Antagonists & Agonists > DAF-FM DA
DAF-FM DA

CAS:254109-22-3

Appearance:White to off-white solid

Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months

Purity:≥98%

   Manual Download
DAF-FM DA
Cat.No：
ID3250
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
ID3250-50ug Inquiry Inquiry Inquiry Inquiry
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Product Information Related Products Related Paper COA Technical Q&A

DAF-FM DA is a membrane permeable nitric oxide (NO) fluorescent probe with Ex/Em=495/515nm. DAF-FM DA actively diffuses through the cell membrane, and once inside the cell, intracellular esterase deacetylates it to form DAF-FM. DAF-FM itself is very weak, but the fluorescence is enhanced by about 160 times after reaction with NO.

Procedure (for reference only)

1. Prepare live cells on cell suspension or slide;

2 Dilute the DMSO storage solution with an appropriate buffer or fresh medium (not serum and phenol red). The recommended initial working concentration range is 1-10μM.

3 The cells were hydrolyzed with the diluted DAF-FM DA working solution for 20 to 60min, and the incubation temperature was 4 to 37℃. Adherent cells do not need to be digested and reloaded.

4 Thoroughly wash cells with PBS, pH 7.4 until excess probes are removed. Replace the fresh slow medium solution or medium and incubate it for 15-30min, so that the lactase can fully de-esterify the probe.

5 The maximum excitation and emission wavelengths are 495 and 515nm, respectively. Because these wavelengths are very similar to fluorescein, individual fluorescein or FITC detection systems are suitable for each.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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