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Feulgen Nucleus Stain Kit

G3070-241212Manual

Cat.No：G3070 Solarbio

Storage：Store at 2-8℃，avoid light，6 months

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Product Information

Name	Feulgen Nucleus Stain Kit
Storage	Store at 2-8℃，avoid light，6 months
Delivery Time	2-3 days
Unit	Kit
Specification	3*50ml

Product Introduction:

The methods of DNA staining include Feulgen method, Methyl Green-Pyronin method, Acridine Orange Fluorescence method and so on. The most classical one is Feulgen method, which is a classical enzyme histochemistry method.
The principle of this kit is that after DNA is hydrolyzed by mild weak acid (such as hydrochloric acid), the glycosidic bond between purine base and deoxyribose is opened, and the phosphoester bond between deoxyribose and phosphoric acid is broken, forming free aldehyde group at one end of deoxyribose. The aldehyde group combines with Schiff Reagent in situ to form a purplish red compound, which makes the part containing DNA in the cell show purplish red. The reason of purplish red is that there is a quinone group in the molecule of the reaction product (the quinone group is a chromophore with color, so the part containing DNA is purplish red). This hydrolysis does not affect the ribose-purine binding bond, so RNA will decompose after being treated with this method, so this method is not applicable to prove RNA.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.