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CAS:7240-37-1
Appearance:Soild
Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
Purity:≥98%
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7-AAD is a non-permeable fluorescent nucleic acid dye. The dye cannot penetrate the cell membrane of living cells, but can penetrate the cell membrane of membrane damaged cells such as late apoptotic cells or necrotic cells and bind to their DNA. It can be used to distinguish between surviving early cells and necrotic or late apoptotic cells, and is widely used in flow cytometry.
7-AAD can emit strong fluorescence after binding with DNA, and its fluorescence characteristics are similar to PI, and it can be excited by 488 nm argon ion laser, but its emission spectrum is narrower than PI, and the emission wavelength is longer, and the interference to other detection channels is less. It is the best substitute for PI in multicolor fluorescence analysis, and can be used in combination with a variety of fluorescent dyes excited by 488 excitation light. Such as FITC, PE, etc.
Method of use (for reference only)
Usually the 7-AAD stain is a step after the cell stain, and it can only stain dead cells. The following procedures apply to most cell types. Growth medium, cell density, cell type, and other factors may affect staining. Residual detergents on glassware may also affect the staining of many organisms, resulting in the appearance of brightly stained substances in solutions where no cells are present.
1) Use a fixed solution suitable for your sample.
2) The cells were precipitated by centrifugation and resuspended in a buffer salt solution or medium for dye staining at pH 7.4. Adherent cells in the culture can be stained locally on the cover slide or in cell culture pores.
3) 7-AAD was added with a concentration of 0.5 to 5μM, and incubated for 15 to 60 minutes.
4) Measurement of fluorescence intensity at Ex/Em =545/650nm (flow cytometry using FL3)
Caution: 7-AAD is a potential carcinogen. Users are advised to wear gloves, protective clothing and eye/face protection to avoid contact with skin and eyes.
