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Product Introduction:
Mucin Staining Kit (Mild Methylation Method) is a type of chemical modification and blocking method, which is based on the principle of eliminating the basophilicity of COOH by using an acidic alcohol solution.
Alcian staining solution at pH 2.5 ionizes the carboxyl groups within the tissue with a negative charge, forming a salt bond with the cations in Alcian blue, which stains tissues with carboxyl groups (e.g., proteoglycans/hyaluronic acid, and epithelial acidic mucins), and is primarily used to identify acidic groups in mucins. Untreated mucin sulfate, proteoglycan sulfate, salivary mucin, and hyaluronan stain blue, and treated sections stain light blue.
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未处理的硫酸黏蛋白、硫酸蛋白多糖、唾液粘蛋白、透明质酸着蓝色，处理后仅硫酸化粘液质着淡蓝色
